Background: Youth friendly health principle is an important mode of delivery of adolescent health services. Objective: To verify the impact of sensitization on reproductive and sexual health issues of adolescent clients in clinic setting using the youth-friendly health principles. Design: An urban–based interventional study. Setting: Urban-based adolescent health clinic. Study Population: School-going adolescents in the age group of 14-18 years (both boys and girls). Intervention: Youth-friendly sensitisation. Main outcome measures: Change in the knowledge on different reproductive and sexual health issues among adolescent clients. Result: Statistically significant improvement (p values< 0.05) in knowledge on various aspects of reproductive and sexual health issues among adolescent clients. Conclusion: Youth-friendly principle is an important tool for making positive changes in the knowledge of adolescents on reproductive and sexual health issues.